CultureOne
Alfa Laval CultureOne™ is a premium range of separators designed for single-use bioprocessing. With proven innovations for gentle product handling and enhanced yield in cell harvesting, CultureOne™ delivers unmatched separation efficiency for high-density cell cultures in biopharmaceutical processing.
Single-use centrifuges for improved cell harvesting
- Proven product recovery yields of up to 99% in single-use bioprocessing
- Easily scalable to larger Alfa Laval stainless steel separators
- 50 to 80% less water, energy, and cleaning agents compared to filter-based harvesting methods – with only a fraction of plastics
- Faster harvesting at large volumes and high cell densities
- Compact design and fast batch changeover
With Alfa Laval CultureOne™ separators, you can maximize efficiency in single-use bioprocessing, ensuring optimal results from your cell harvesting processes. These separators deliver higher product yields compared to other single-use centrifuge technologies, with proven recovery rates of up to 99%. The Hermetic Design of the CultureOne™ separator ensures gentle treatment, reducing cell lysis and avoiding foaming—making downstream purification more effective and reliable for biopharmaceutical processing.
Featuring Alfa Laval Spinsert™, our patented single-use disc stack, these separators enable quick setup and replacement, ensuring fast batch changeovers and seamless integration into single-use bioprocessing workflows.
With superior separation performance, CultureOne™ separators provide a clear centrate with minimal remaining solids content, significantly reducing the need for secondary filtration and lowering the ecological footprint.
Designed for ease of use, CultureOne™ separators can operate as stand-alone units or as part of a centralized system. Their advanced automation and connectivity ensure complete data integrity, fully compliant with 21 CFR Part 11, meeting the rigorous demands of biopharmaceutical processing.
Proven and published performance in biopharmaceutical processing
BioProcess International published a study detailing the performance of CultureOne Primo. Made in collaboration with Genentech, the study shows that the single-use centrifuge gives a recovery yield rate of up to 99% in cell harvesting and single-use bioprocessing.
The CultureOne range
CultureOne™ separators enhance efficiency in single-use bioprocessing, increasing product recovery and streamlining workflows from R&D to production scale.
CultureOne Primo
- Processing capacity: 240 litres/hour
- Product recovery yield: Up to 99%
CultureOne Maxi
- Processing capacity: 720 litres/hour
- Product recovery yield: Up to 99%
* Capacity figures for fed-batch mammalian cell culture materials. Actual process performance is a function of individual material characteristics.
How it works
Watch: How CultureOne™ enhances cell harvesting in biopharma
Curious about the CultureOne™ separators? In this short animation, you can explore how these advanced systems support cell harvesting.
Main facts about CultureOne™
Improving yields at every scale
The CultureOne separation platform is designed to maximize the potential of every cell culture, delivering unparalleled yields, ensuring product quality with utmost security, and minimizing additional lysis. The range ensures consistent performance throughout the transition from R&D to full-scale production, allowing for seamless scalability.
Developed with the industry to meet the most challenging demands
The CultureOne separation system was developed in close collaboration with leading biopharmaceutical manufacturers. Its unique performance has been verified in a wide range of processes to ensure it can meet ever-changing demands.
Less waste in single-use processing
With CultureOne separators, you can reduce the amount of water, chemicals, and plastic consumables required for single-use biopharma processing by 50 to 80%. The result is lower costs and added sustainability.
Are you considering single-use centrifugation?
Why switch to single-use processing?
A single-use separation system secures patient safety by eliminating the risk of cross-contamination between batches. Single-use processing also enables a reduced footprint and increased flexibility, with no need for sterilization: empowering manufacturers to bring new treatments to market faster than ever before.
A better harvest with centrifugal separation
Many single-use harvesting systems cannot efficiently separate high-density cell cultures. The resulting product loss dramatically impacts the productivity and profitability of biopharmaceutical operations. CultureOne overcomes this problem by combining high-efficiency centrifugal separation with secure and convenient single-use processing.
Safety and quality with Spinsert™
CultureOne's performance is based on the Spinsert, a patented single-use disposable bowl and disc stack assembly – fully sterilized and ready for processing. Easy to set up and replace, it ensures fast batch changeover. In order to achieve this, Alfa Laval has integrated proven technology from stainless steel separators:
- Hermetic Design, an Alfa Laval unique design, providing low shear, higher product recovery, and foam free separation
- TopStream™ enables continuous concentrated solid ejection during separation
- UniDisc™ increases disc area for improved separation efficiency.
Get the answers you need
Visit the Q&A section for answers to some of the most common questions about CultureOne and its potential – or send your question to our experts.
Services
Extending the performance of your equipment
Our dedicated global service network supports you with parts and expertise, wherever you are, whenever you need it. By ensuring greater uptime, availability and optimization, we help bring you peace of mind, and maximize your return on investment. We can work with you to integrate our services into a Performance Agreement for predictable and worry-free operations.
The Separator Innovator
Would you like to know more about our innovations in separation technology? Click on the link below. Alfa Laval invented the first disc stack separator and has led the way in the development of centrifuge technology for over a century. We are glad to share with you the expertise we have gained along the way. Visit our Separator Innovator knowledge base to learn more about advancements in separation and the steps Alfa Laval is taking to continue revolutionizing the technology.
Test, validate, and scale up your next separation solution
Whether upgrading your production line or running a proof of concept, Alfa Laval helps you choose the right separator solution with confidence. Simply get in touch to arrange a consultation and trial at one of our test centres, or to rent a unit for onsite testing.
